Modular computer programs for flow cytometry and sorting: The LACEL system
نویسندگان
چکیده
منابع مشابه
Application of flow cytometry and cell sorting to megakaryocytopoiesis.
We have employed flow cytometry (FCM) and cell sorting to quantitate and study megakaryocytes in mouse and rat femoral marrow following their 20- to 30-fold concentration by centrifugal elutriation (CE). This enrichment of megakaryocytes permitted the first determination of their DNA-related fluorescence by FCM analysis following DNA staining. Fluorescence distributions of CE-enriched cell frac...
متن کاملFlow Cytometry-Based Analysis and Sorting of Lung Dendritic Cells
Dendritic cells (DC) are very specialized antigen presenting cells which play critical roles in innate and adaptive immunity and in tolerance. Their major functions are to sample antigens throughout the body and to stimulate or tolerize T cells. DC consist of several distinct subsets with different tissue distribution, surface markers, and function. This chapter discusses the lung DC subsets, t...
متن کاملUltrahigh-throughput sorting of microfluidic drops with flow cytometry.
The detection and sorting of aqueous drops is central to microfluidic workflows for high-throughput biology applications, including directed evolution, digital PCR, and antibody screening. However, high-throughput detection and sorting of drops require optical systems and microfluidic components that are complex, difficult to build, and often yield inadequate sensitivity and throughput. Here, w...
متن کاملA Transformation System for Modular CLP Programs
We propose a transformation system for CLP programs and modules. The framework is inspired by the one of Tamaki and Sato for pure logic programs 19]. Here, the use of CLP allows us to introduce some new operations such as splitting and constraint replacement. We provide two sets of applicability conditions. The rst one guarantees that the original and the transformed programs have the same comp...
متن کاملthe search for the self in becketts theatre: waiting for godot and endgame
this thesis is based upon the works of samuel beckett. one of the greatest writers of contemporary literature. here, i have tried to focus on one of the main themes in becketts works: the search for the real "me" or the real self, which is not only a problem to be solved for beckett man but also for each of us. i have tried to show becketts techniques in approaching this unattainable goal, base...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry
سال: 1981
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990010505